- Visibility 220 Views
- Downloads 31 Downloads
Impregnation and embedding using bees wax and paraffin wax in oral tissue: a comparative study
- Author Details:
-
J. Vini Mary Antony
-
Pratibha Ramani
-
Anuja N
Paraffin wax is normally used in impregnation and embedding of tissue samples in biopsy. This study aims to compare paraffin wax that is used as routine embedding media and beeswax in impregnation and embedding of tissues. 10 biopsy specimens were impregnated and embedded in paraffin wax and 10 biopsy specimens were impregnated and embedded in beeswax. After manual processing all sections were stained with Haematoxylin and Eosin (H and E) to compare the effect of beeswax and paraffin wax based on the features of integrity of the section, uniformity of the stain, staining which includes nuclear details, cytoplasmic details and the background staining. The study findings proved that beeswax showed well impregnation and embedding of the tissues as well as preservation of the nuclear details, good cytoplasmic appearance, good tissue architecture and no bad effect on staining characteristics of the tissue. In addition, beeswax reduced the time needed for wax cooling. We conclude from our study that beeswax work well in preparation of tissue specimen when compared to paraffin. Thus, we recommended using beeswax in our laboratories as national available waxes.
Keywords: Bees wax, Paraffin, Embedding, Impregnation
References
- Sanford, M.T.; Dietz, A. (1976). "The fine structure of the wax gland of the honey bee (Apis mellifera L.)". Apidologie. 7:197–
- doi:10.1051/apido:19760301
[Google Scholar] - Brown, R, H. (1981) Beeswax (2nd edition) Bee Books New and Old, Burrowbridge, Somerset UK. ISBN 0- 905652-15-0.
- Beeswax Production, Harvesting, Processing and Products, Coggshall and Morse. Wicwas Press. 1984-06-
- ISBN 1878075063.
- Les Crowder (2012-08-31). Top-Bar Beekeeping: Organic Practices for Honeybee Health. Chelsea Green Publishing. ISBN 1603584617.
- Top-bar beekeeping in America.
- Umney, Nick; Shayne Rivers (2003). Conservation of Furniture. Butterworth-Heinemann. p. 164.
- "LIPID MAPS Databases: LIPID MAPS Lipidomics Gateway". Lipidmaps.org. Retrieved 2013-07-05.
- A Dictionary of Applied Chemistry, Vol. 5. Sir Edward Thorpe. Revised and enlarged edition. Longmans, Green, and Co., London, 1916. "Waxes, Animal and vegetable. Beeswax", p. 737.
- 'Metal Injection Molding Process (MIM)", 2012 Eng Pedia.
- Congdon, L. O. K. (1985). "Water-Casting Concave- Convex Wax Models for Cire Perdue Bronze Mirrors". American Journal of Archaeology. 89(3):511–
- JSTOR 504365.
- Ormeling, F. J. 1956. The Timor problem: a geographical interpretation of an underdeveloped island. Groningen and The Hague: J. B. Wolters and Martinus Nijhoff.
How to Cite This Article
Vancouver
Antony JVM, Ramani P, N A. Impregnation and embedding using bees wax and paraffin wax in oral tissue: a comparative study [Internet]. J Oral Med Oral Surg Oral Pathol Oral Radiol. 2017 [cited 2025 Oct 11];3(1):42-44. Available from: https://doi.org/
APA
Antony, J. V. M., Ramani, P., N, A. (2017). Impregnation and embedding using bees wax and paraffin wax in oral tissue: a comparative study. J Oral Med Oral Surg Oral Pathol Oral Radiol, 3(1), 42-44. https://doi.org/
MLA
Antony, J. Vini Mary, Ramani, Pratibha, N, Anuja. "Impregnation and embedding using bees wax and paraffin wax in oral tissue: a comparative study." J Oral Med Oral Surg Oral Pathol Oral Radiol, vol. 3, no. 1, 2017, pp. 42-44. https://doi.org/
Chicago
Antony, J. V. M., Ramani, P., N, A.. "Impregnation and embedding using bees wax and paraffin wax in oral tissue: a comparative study." J Oral Med Oral Surg Oral Pathol Oral Radiol 3, no. 1 (2017): 42-44. https://doi.org/